Not convinced by the smaller bubbles but the solubility argument is interesting. What's the relationship between dissolved gas content and compressibility? I was wondering why professionals use negative pressure, and we use positive pressure. But that could be a different trade-off between capital cost and labour time rather than improved outcome. (Crossed with Oliver's costing.) Jonathan PS: I'm cautious about attributing superior performance to the last used method. See also finding keys in the last place you look. PPS: I worked with bubbles in two very different ways in my career. Bubble management was the technological breakthrough that led to the Technicon AutoAnalyzer. And that's what allowed cheap, widespread multichannel clinical chemistry analyses that most of you will have used as patients. It was eventually beaten by pre-existing conventional liquid-only systems that replicate manual techniques using pipettes and cuvetttes.